X-ray Irradiation Facilities

Overview of Services

X-ray irradiators for cells: Two CIX2 X-ray cabinets from Xstrahl have been acquired. One is localised at Campus Solna and one at Campus Flemingsberg. They are 220kV self-contained cabinet irradiators. The primary use of the cell irradiators is to irradiate cultured cells suspended in cell culture medium in 15 ml and/or 50 ml centrifugation tubes or growing in tissue culture vessels as a mono-layer in sizes up to 175 square-centimeter cell culture bottles or different type of multi-well plates i.e. 6-well up to 384-well plates.
X-ray irradiators for small animals: Two CIX3 X-ray cabinets from Xstrahl have been acquired and are available at KM-A and KM-B. They are 320kV self-contained cabinet irradiators. The cabinets can be used for whole body irradiation (KM-A, KM-B) or focal irradiation (KM-B only).

Xstrahl’s CIX Series cabinet irradiators are free-standing, self-contained X-ray irradiators, designed to facilitate the safe and accurate irradiation of biological samples within a standard laboratory environment. Fulfilling the requirements of in vitro and in vivo biological research, the CIX2 and CIX3 are 220kV and 300kV respectively self-contained cabinet irradiators incorporating the irradiation chamber and system electronics in one enclosure. The intuitive and easy to use software interface allows for multiple user logins, whilst X-Ray exposures can be programmed and executed automatically. In addition to removing the health and safety burden associated with the use of radioactive sources, the Xstrahl CIX2/3 provide a simpler, safer and lower cost alternative to radioisotope irradiators. Research Applications:
• In vitro irradiation
• In vivo irradiation
• Radiobiology studies
• DNA repair mechanisms
• Tumour micro environment
• Bystander effects
• Normal tissue toxicity
• Low dose radiobiology studies • Hypoxia research
• Combination therapy studies
• Radioimmunotherapy
• Immunology
• Bone marrow chimeras 

Access, Booking and fees

Open for external users?
Yes

Training and user fee?
A training and user fee will be applied for using the X-ray irradiators.

Education mandatory before access to equipment?
Yes. It is a legal requirement that you have attended one course on "Introduction to radiation protection" prior to start using the X-ray irradiators and also a web course focused on the “Xstrahl X-ray irradiators”. In addition, you need to attend an onsite introduction to the irradiators.
You should have participated in the "Introduction to radiation protection" course/session no longer than five years ago. Please provide a certificate when contacting the experts for access. If your certificate is older than five years or if you have not participated in such a course previously, you must register for one. See further details for registration below.
If you have attended an equivalent course, you need to provide a certificate and sign up for an onsite introduction to get access to the X-ray irradiator. See contact details below.

Registration for the "Introduction to radiation protection" and “Xstrahl X-ray irradiators” courses
The courses are offered through the Nano Learning platform via the page Radiation Safety on the Staff Portal.

Registration for the Onsite Introduction
Please register your interest in attending the introduction by sending an email to Adamantia Fragkopoulou (adamantia.fragkopoulou@ki.se) and a training request through iLab.

Locations and hours of operation

Links and Resources

1. X-ray Irradiation Core Facility - nodes ANA Futura & Biomedicum | Karolinska Institutet (ki.se)
2. X-ray Irradiation Core Facility (nodes KM) - our offer | Karolinska Institutet (ki.se)

Contacts