Eukaryotic Single Cell Genomics Facility

Overview of Services

Single-cell sequencing and single-cell RNA-sequencing technologies have proven to give new insights into cell type discovery and in the characterisation of heterogeneity in tumors as well as in normal tissue.  
 
Eukaryotic Single Cell Genomics (ESCG) facility aims at providing high-throughput single cell transcriptomic services to the Swedish and the international scientific community. We offer streamlined single-cell RNA sequencing at full-service. The users provide us with single cells, we process the samples, sequence and deliver annotated gene expression data.

Applications

  • Study heterogeneity within putatively homogenous cell populations
  • Unbiased discovery of cell types in complex tissues
  • Characterizing the cellular and genetic composition of tumors 

Services 

  • Single-cell RNA-sequencing
  • Single-nucleus RNA-sequencing
  • Single-cell ATAC-sequencing
  • Single-cell Immune Profiling 
  • Single-cell CUT&Tag
  • CITE-seq and cell/nuclei hashing
  • Single-cell CRISPR screens in collaboration with the HTGE facility
  • Sequencing; We sequence our single-cell libraries with NGI Stockholm
  • Bioinformatics; We refer to NBIS for project specific bioinformatic analyses 

Methods

  • Smart-seq3, full-length RNA-sequencing (plate-based, 384 cells)
  • 10x Genomics (droplet-based platform)

 

Location and hours of operation

Hours Location

Monday - Friday  

9:00 - 17:00h

Science for Life Laboratory

ESCG, Gamma 4

Tomtebodavägen 23A

171 65 Solna

 

Delivery address

Please don't submit any samples without prior agreement with the facility.

 

Science for Life Laboratory

ESCG, Gamma 4

Tomtebodavägen 23B

171 65 Solna

 

Links and Resources

  1. Eukaryotic Single Cell Genomics SciLifeLab site
  2. ESCG Publications

Contacts

Name Role Phone Email Location
ESCG
Core email
 
08-524 85266
 
escg@scilifelab.se
 
Gamma 4
 
Karolina Wallenborg
Head of Unit
 
08-524 81242
 
karolina.wallenborg@scilifelab.se
 
Gamma 4