Eukaryotic Single Cell Genomics Facility

OBS!

From October 1st 2024, NGI/ESCG will no longer be accepting project orders through the iLab system. To submit an order for a single-cell project with NGI/ESCG, please instead go to the NGI order portal (here: https://ngisweden.scilifelab.se/orders/) and fill in the Single-cell sequencing order form.

Please contact us at support@ngisweden.se if you have any questions about this. Please also contact us BEFORE submitting an order if you want to start with a general discussion about the options for single-cell sequencing with us at NGI.

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Overview of Services

Single-cell sequencing and single-cell RNA-sequencing technologies have proven to give new insights into cell type discovery and in the characterisation of heterogeneity in tumors as well as in normal tissue.  
 
Eukaryotic Single Cell Genomics (ESCG) facility, a part of the National Genomics Infrastructure (NGI), aims at providing high-throughput single cell transcriptomic services to the Swedish and the international scientific community. We offer streamlined single-cell RNA sequencing at full-service. The users provide us with single cells, we process the samples, sequence and deliver annotated gene expression data.

Sequencing platforms

• NovaSeq X Plus
• NextSeq 2000

Applications

• Study heterogeneity within putatively homogenous cell populations
• Unbiased discovery of cell types in complex tissues
• Characterizing the cellular and genetic composition of tumors 

Services 

• Single-cell RNA-sequencing
• Single-nucleus RNA-sequencing
• Single-cell ATAC-sequencing
• Single-cell Immune Profiling 
• CITE-seq and cell/nuclei hashing
• Single-cell CRISPR screens in collaboration with the CFG facility
• Sequencing; We sequence our single-cell libraries with NGI Stockholm
• Bioinformatics; We refer to NBIS for project specific bioinformatic analyses 

Methods

• Smart-seq3, full-length RNA-sequencing (plate-based, 384 cells) (new projects until December 2024)
• 10x Genomics (droplet-based platform)

Location and hours of operation

Delivery address

Please don't submit any samples without prior agreement with the facility.

Science for Life Laboratory

ESCG, Gamma 4

Tomtebodavägen 23B

171 65 Solna

Links and Resources

1. Eukaryotic Single Cell Genomics SciLifeLab site
2. ESCG Publications

Contacts