Single Cell Proteomics

Overview of Services

Single Cell Proteomics is the proteomics core facility at the Department of Medical Biochemistry and Biophysics located in the Biomedicum building. We provide a broad range of services using best available proteomics techniques and methods with a focus on quantitative proteomics, both label-free and multiplexed TMT-labeling for Single-Cell and low-protein-amount samples, such as Embryos.

Based on our many years of experience, we are always glad to discuss projects related to the identification and quantification of proteins, assist with experimental design, and deliver high-quality results.

We are continuously working on the adaptation of different Chemical Proteomics techniques to the Single-Cell level. Currently, we offer Functional Identification of Target by Expression Proteomics (FITExP) which we have shown its application on Single Cells (SCCP).

Services

• Single-cell proteomics by mass spectrometry
• Single-cell chemical proteomics (SCCP)
• SP30-SP40 DIA approach for fast and reliable proteome expression on Single cell
• Large-scale quantitative proteomics experiments using label-free or isobaric labeling (TMT and TMTpro) quantification strategies
• Detection of posttranslational modifications and their localization on the amino acid sequence

Operating Guidelines

From October 1, 2020 the following guidelines are introduced:

After discussion but before submission of samples, the customer is kindly requested to provide a brief description of the project. The customer would need to choose (and/or) different levels of services:

• Contract (service) analysis (Research data acquisition. No data analysis or interpretation will be provided by facility. Archiving and public access is customer’s responsibility. No intellectual property will be retained by the facility. No authorship demand by facility. User fee will be applied.)
• Collaborative analysis (Besides research data acquisition (for which the same user fee will be applied as in Contract (service) analysis), data analysis and interpretation will be provided. Archiving and public access will be provided. Intellectual property will be shared & authorship will be shared (see Rules on Intellectual Property and Publications for details).

Single Cell Proteomics is also happy to be engaged with Collaborative research, when the facility is part of the grant application together with collaborator(s) and develop new analytical methods applicable to collaborator’s analytical problem.

Personnel

Roman A Zubarev, PhD – facility director

Hassan Gharibi, PhD - core facitliy manager

Marie Ståhlberg - technical expert

Zhaowei Meng – LC-MS engineer

Mauricio Quiñones, PhD - Postdoctoral Researcher

Location and hours of operation

Links and Resources

1. Single Cell Proteomics Core Facility webpage

Contacts