Autoradiography Core Facility

Overview of Services:

The autoradiography facility (ARG) is a core facility for radioligand binding and autoradiography, critical components in pharmacology and cellular signaling research, as well as for development of ligands for usage in positron emission tomography (PET).

Autoradiography is a method to visualize and quantify the binding of radioactive molecules in tissue sections and is commonly used for mapping of G protein coupled receptors (GPCRs) and transporters in brain from pathologic conditions and in animal models. The method is extensively applied as a tool for validation of novel radioligands for PET since it provides high resolution image correlates that may aid the interpretation of data obtained in PET studies. Moreover, an initial characterization of tracer affinity, selectivity, and non-specific binding using autoradiographic methods in vitro may predict suitability of the tracer application in vivo.

Equipment:

• Phosphoimager: GE Typhoon FLA9500 (and eraser)
• Liquid Scintillation Counter: Hidex 600SL
• Plate filtering and separation system: Millipore MultiScreen
• Lab bench and Chemical safety hood, for isotope work
• Cryostats:  Leica CM1860
• Safety equipment: Our facility has tabletop beta and gamma shields, led aprons and a led shielded waste container.

 Isotopes licensed:

3H, 35S, 125I, 11C, 18F, 68Ga, 89Zr, 64Cu, 99mTc

 

 

 

Location and hours of operation

Hours	Location
Monday - Friday    8:30 - 18:00h	Bioclinicum, J5:20 Akedemiska Stråket 1 Solna 171 64

 

Contacts

Name	Role	Phone	Email	Location
Vasco Sousa	Facility manager		vasco.sousa@ki.se	Bioclinicum, J5:20
Åsa Södergren	Researcher		Asa.sodergren.1@ki.se	Bioclinicum, J5:20

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