About

MedH Flow Cytometry Core Facility

About us

Flow cytometry is a central technology in biomedical research allowing multiparametric analysis and isolation (by cell sorting) of rare cell populations down to a single cell from complex cell mixtures.
The Department of Medicine Huddinge (MedH) flow cytometry core facility is a service facility located at the 7th floor of the NEO research building at the Karolinska Huddinge Campus (see visiting address). The MedH FACS facility provides open access to all interested researchers at KI and Karolinska Hospital regardless of their affiliation and strives to provide each user with skilled services, education and training to achieve high quality research.

Established in 2011 with an initial support from the Wallenberg Institute for Regenerative Medicine (WIRM), a research institute financed by Knut och Alice Wallenbergs Stiftelse, the facility is currently supported by core facility funding from KI/SLL and Stratregen grant from Karolinska Institutet. To cover most of its running costs, the facility charges fees for its services (see user Fees for detailed information).

If you need additional information about services/technology, please contact us via email at one of the below addresses.

 

Biosafety and Risk assessment

A risk assessment shall be carried out in order that everyone shall have a safe work environment, and to raise risk awareness. Prior to cell sorting, the facility requires individual risk assessment for each new project (under the "Request Services")

In addition, the Biosafety Committee at KI has created a risk assessment form for microorganisms, BARA (Biological Agents Risk Assessment). The form can be used to assess the risk of handling micro-organisms (including cell cultures). For risk assessment of blood and other human sample material, the Biosafety Committee has created a form HUMRA (Human Sample Material Risk Assessment).

  • Note that the BARA form should not be used for microorganisms that have been genetically modified, refer instead to ’GMM-applications’.
  • Notification/permit is required for all microorganisms in risk group 2 and higher. Also refer to 'Applications to handle microorganisms'.
  • When relevant assessing the risk of chemicals, refer instead to ’Risk assessment – Chemicals’

 

Booking Rules: Analysers (Fortessa, Symphony, CytoFlex)

  • Booking max 3 weeks in advance
  • Booking can be cancelled or modified up till 24 hrs before analysis (locked and charged as it is after 24hr cutoff)
  • Block bookings can be made for max 3 hrs between 08.00 -16.00 and 5 hrs otherwise. If the instrument is free, longer bookings can be made with the obligation to be flexible to other users who might need to use the machine.
  • Minimum booking time 1 hr
  • Override possible with justification and interaction with facility manager


Booking Rules: Sorters (Arya and Sansa)

  • Only independent users have direct access to the booking calendar
  • Booking max 3 weeks in advance
  • Booking can be cancelled or modified up till 24hrs before analysis (locked and charged as it is after 24hr cutoff)
  • Block bookings can be made for max 8 hrs
  • Minimum booking time 1 hr 
  • Override possible with justification and interaction with facility manager
 

 Requirements to be a sorter independent user

  • Proven/certified external training with experts in the field *
  • Extensive period of training (at least one week) *
  • Instrument startup-shutdown on site with supervision (at least 3 times)
* BD training or equivalent

 

Location and hours of operation

Hours Location

Monday - Friday   

8:00h - 16:00h  

MedH Flow Cytometry Facility

Neo Research Building, floor 7 (7N)

Blickagången 16, SE-141 83 Stockholm

 

Useful Links

 
 
 
 

More Flow Cytometry Links:

http://www.cyto.purdue.edu/hmarchiv/index.htm

http://isac-net.org/

BD spectrum viewer

Biolegend spectra analyzer

Lifetechnologies fluorescence spectra viewer

https://www.bdbiosciences.com/en-se/resources/scientific-resources?resourceType::Video=Video

Contacts

Name Role Phone Email Location
Belinda Pannagel
Facility manager
 
+46 8 524 83989
 
belinda.pannagel@ki.se
 

 
MedH FACS Core Facility

 

 
facs@medh.ki.se
 

 
Managers Office

 
08524 83458
 

 

 
Narmadha Subramanian
Facility manager
 

 
narmadha.subramanian@ki.se
 

 

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